Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/28/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Chelmsford High School |
| Observations | The arthropod was caught near the science pond. It was very small, brown in color, and had delicate wings. |
| Putative identification | Arthropoda Insecta Diptera Drosophilidae Drosophila Drosophila suzukii |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | agarose gel electrophoresis |
| Buffer | 1X TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | My confidence level is medium because although the controls were successful, the well containing the arthropod may not have been completely filled, causing Wolbachia not to be detected. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Drosophila suzukii was found to be negative for Wolbachia. |
CHS A.S. 5/28/2026 – 5
CHS A.S. 5/28/2026 – 4
CHS A.S. 5/28/2026 – 3
CHS A.S. 5/28/2026 – 2
CHS A.S. 5/28/2026 – 1