Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 06/01/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Winston Knoll Collegiate |
| Observations |
|
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius neoniger |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR's BlueGel |
| Buffer | 1X TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Confidence level is medium because one of our two positive controls showed a band. Our negative control behaved as expected and did not show a band. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lasius neoniger was found to be negative for Wolbachia. |
This arthropod was found close to water, relative to our other samples. It did not want to surface and insisted on returning to the soil. It had a reddish / brown appearance and was very active (it was moving a lot when we collected it). It was found in a patch of dirt in a field of grass.
WKC – CAKA – Wascana Dryest Dry 2
WKC – CAKA – Wascana Dryest Dry 1
WKC – CAKA – Wascana Dry 2
WKC – CAKA – Wascana Dry 1
WKC – CAKA – Wascana Wet 2