WKC – Ariq C – Wascana Wet 2 – Lasius Neoniger

June 15, 2026

Sample information
Picture
Entry by: Ariq Chauhan
Location
Collection date 06/15/2026
Captive / Cultivated? Wild-caught
Group Winston Knoll Collegiate
Observations

This arthropod was found close to water, relative to our other samples. It did not want to surface and insisted on returning to the soil. It had a reddish / brown appearance and was very active (it was moving a lot when we collected it). It was found in a patch of dirt in a field of grass.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius neoniger

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR's BlueGel
Buffer 1X TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Our confidence level is high because all of our controls worked as expected. In our arthropod gel, all of the wells showed a band except for the arthropod negative control, as expected. In our Wolbachia gel, our positive controls showed a band, whereas our negative controls did not, as expected. We are confident in the diagnosis of this arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lasius neoniger was found to be negative for Wolbachia.
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