Firefly 2026

October 15, 2026

Sample information
Picture
Entry by: Izzy S.
Location
Approximate location: 36.01, -84.27
Collection date 09/09/2026
Captive / Cultivated? Wild-caught
Group Oak Ridge High School
Observations

I captured this firefly in my backyard (wooded area) around dusk.
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain SeeGreen
Gel images
Protocol notes

I followed protocol closely and accurately according to the instructions I was given.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident that my arthropod is Wolbachia negative because I followed protocol closely. The line showed in my lane was strong and the controls worked too.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Coleoptera was found to be negative for Wolbachia.
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