Sample information
Picture
Location	Approximate location: 36.02, -84.26
Collection date	09/08/2026
Captive / Cultivated?	Wild-caught
Group	Oak Ridge High School
Observations	\
Putative identification	Arthropoda Insecta Hemiptera Cicadellidae Agallia Agallia constricta
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniPCR
Buffer	1X TBE
DNA stain	Cyber Green
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	The results that came back indicated strong results.
Wolbachia 16S sequence	Download AB1
Arthropod COI sequence	Download AB1
Summary	The Agallia constricta was found to be postive for Wolbachia.