Sample information |
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| Picture |
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|---|---|
| Location | Approximate location: 31.97, -81.16 |
| Collection date | 08/31/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | The insect was collected in a grassy, wet area with a sweep net by sweeping the grass. |
| Putative identification | Arthropoda Insecta Coleoptera |
Methods |
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| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
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| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | The gel electrophoresis sequences showed the presence of Wolbachia; however, they show additional bands, so the final result is unclear. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
ATATTAGGCGCCCCTGATATAGCATTCCCTCGAATAAA CAATATAAGATTCTGGCTTCTCCCCCCTTCTCTAACCCTTCTTTTAATAAGCAGGCTAGTTGAAAATGGAGCCGGAACTG GTTGAACAGTGTATCCCCCACTGTCAGCTAATATCGCACACAGAGGTGCTTCAGTAGATTTAGCTATTTTTAGCCTTCAT CTAGCAGGAATCAGTTCAATTCTCGGAGCAGTTAATTTTATTACTACTATAATCAATATACGTTCAATAGGCATAACATT TGACCGAATACCCTTATTTGCCTGATCAGTTGTACTTACTGCTCTTTTACTTCTCCTATCCCTCCCAGTTTTAGCAGGAG CTATCACAATGCTCCTCACTGATCGTAATATCAACACAACATTTTTTGACCCTGCGGGANGANGAGACCCAATTCTATAC CAACACTTATTTTGATTTTTTGGT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
CON-MCH
CON-BJA Hymenoptera
CONKDLH-orthoptera
CON-HAS Hymenoptera
CON-AEP Hymenoptera