CHI-HDT

November 3, 2026

Sample information
Picture
Entry by: Harley T
Location
Approximate location: 31.97, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Overgrown garden, tall grass
Putative identification Arthropoda Insecta

Methods
Extraction kit tweezers
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I feel confident I got good results.
Wolbachia 16S sequence
Arthropod COI sequence
TGGNTCCTCAATAAGAATGATTATCCGTCTAGAATTAGGGACATG TGGATCACTTATTAATAACGATCAAATCTACAACTCAATTATTACAGGTCACGCCTTCATCATAATTTTTTTTATAGTAA TACCATTCATGATTGGGGGATTCGGAAACTTCTTAGTGCCATTAATGATCGGATCTCCTGATATAGCATACCCTCGAATA AATAATATAAGATTTTGACTTCTACCCCCATCAATTATACTTTTAACCCTTAGTAGATTTATTAATACAGGGGTAGGTAC AGGATGAACAGTATATCCACCCCTAGCATCCAACATATTCCATGGAGGGGCATCTGTAGACCTAGCCATCTTTTCTCTTC ATATTGCTGGGATTTCTTCAATCATAGGAGCAATTAATTTTATCTCCACNATTATTAACATAACCCATAAAAATTTCACT ATANATNANGTACCCCTTATAGTCTGGGCTATCTTAATCACCGCTGTTCTTCTATTACTATCACTTCCTGTCTTANCAGG AGCTATTACTATACTTCTAACTGATCGAAATTTAAATACTTCTTTTTTTGACCCTGCTGGTGGGGGAGACCCTATCCTTT TACCAACATTTATTTTGATTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
Report Inappropriate Post