DEM-CJH Blattodea

November 3, 2026

Sample information
Picture
Entry by: Christian H
Location
Approximate location: 31.97, -81.16
Collection date
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught in a humid environment surrounded by pine straw and moist dirt
Putative identification Arthropoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes
  1. dna extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Gel electrophoresis showed no presences of wolbachia and previous data shows low rates of wolbachia presences in blattodea order
Wolbachia 16S sequence
Arthropod COI sequence
GAATATTAATCCGAGCAGAACTAAAT CAACCTGGTTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGGCTCACGCTTTTGTTATAATTTTCTTTAT AGTTATGCCAATTTTAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGCATTTCCTC GTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTCTATCTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCT GGGACTGGTTGAACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGTAGATTTAGCTATTTTCTC ATTACATCTTGCAGGTGTATCATCAATTTTAGGTGCCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATA TAAGCCCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATTATTATTATTATTATCCTTACCAGTTCNT GCANGTGCAATTACAATATTATTAACAGACCGAAATTTANTACTTCATTTTTTGACCCTGCNNNGGANGTGATCNTATTT TATATCAACATTTATTTTGATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattodea was found to be negative for Wolbachia.
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