MATTLM-Diptera

November 3, 2026

Sample information
Picture
Entry by: MATTLM-Diptera
Location
Approximate location: 31.97, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Three diptera were caught in a net on the GSU campus by my biology partner and me.
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My confidence level was low, because my expected outcome was that there would be no evidence of Wolbachia in the specimen. Electrophoresis results prove no Wolbachia was found. The controls work, my partners and I compared the DNA of our specimens with the DNA of a specimen that was infected with Wolbachia. The DNA that contained Wolbachia contained two lines, one being a positive line, while our insects’ DNA only had one line; no Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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