BRI AJC Diptera

November 4, 2026

Sample information
Picture
Location
Approximate location: 31.70, -81.71
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

I caught my insect in my backyard on my mother’s plant. My insect is called a Lovebug and its order is Diptera. When I found it, it was attached to another lovebug at the end.
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

My confidence level was pretty high that my insect would have Wolbachia present. I thought this because the classroom chart said 18% of dipteras have Wolbachia. I was wrong, my insect did not have Wolbachia.
Wolbachia 16S sequence
GTGGCGTGCTGATCCACGATTACTAGCGatcccttcttcgtgCACTCGAGTTGCAGAGTACAATCCGAACTGAgaTggcTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAACCCGGACGAACCGACCCTATCCCTTCGAATAGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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