CONKAE-Orthoptera

November 4, 2026

Sample information
Picture
Entry by: KAE
Location
Approximate location: 31.97, -81.16
Collection date 08/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • August 26th, 2026​
  • Time caught: 1:20 PM​
  • Georgia Southern University, Armstrong Science Building parking lot​
  • Tall grassy area surrounded by high dirt piles and poorly shaded areas​
  • Used Sweep Net​
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit net
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Due to the second band of DNA (the Wolbachia one) seen in the gel lab was much more prominent and was bright green like looking under the UV light.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be postive for Wolbachia.
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