CON-ACS Lepidoptera

November 4, 2026

Sample information
Picture
Entry by: Alicia S
Location
Approximate location: 31.97, -81.16
Collection date 08/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

The insect was caught on a flower outside in my college campus
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I have a second line on my gel, but it’s at the 300 bp mark instead of 400 bp.
Wolbachia 16S sequence
Arthropod COI sequence
ATAGTAGGAACATCTCTTAGTATTTTAATTCGAATAGAATTAGGTAAT CCCGGATCATTAATTGGTGATGATCAAATTTATAATACTATTGTTACAGCTCATGCATTTATTATAATTTTTTTTATAGT TATACCTATTATAATTGGAGGATTTGGTAATTGATTAGTTCCATTAATATTAGGAGCCCCAGATATAGCATTTCCTCGAA TAAATAATATAAGATTTTGACTTCTCCCCCCGTCATTAATTTTATTAATTTCTAGAAGAATTGTAGAAAATGGAGCAGGA ACAGGATGAACTGTTTATCCNCCTCTTTCATCTAATATTGCTCATGGAGGTTCNTCTGTAGATTTAGCTANTTTTTTCCT TACATTTAGCCCGGAATTTCTTCAATTTTANGGGGCAATTAACTTTATTACTACTATNATNAATATACGAATTAATAATA NNTCNTTTNGATCAATTACNNNNNATTNGTTTGANCTGTAGGAATTACNGCNNNNNNNNNNNNNNNNNNCNNNNNCAGTT TTAGCTGGNAGCTATTACTATNCTTTTAACAGATCGAAATTTAAATACATCATTTTTTGATCCNGCTGGAGGGGGAGACC CNATTTTATATCNNCATTTATTTTNATTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lepidoptera was found to be negative for Wolbachia.
Report Inappropriate Post