VIJ-BRI Hymenoptera

November 4, 2026

Sample information
Picture
Entry by: Violet J
Location
Approximate location: 31.97, -81.16
Collection date 09/02/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Date of collection: 9/2/2026
  • Time: 2:43 p.m.
  • Location: 4th Bush from front of parking area behind Science center
  • Tall bush with multiple branches
  • Collection method: Sweep net – Collection jar
Putative identification

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I have high confidence because the controls worked as expected, and my sample showed a Wolbachia band at the expected size. This supports that my insect tested positive for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
AATAATGATNANNTTTACAACTCAATCNTTNNNGCNCATGCNTTAATTATAATTTTTTTTAT NGNTATACNNNNNATNATNGGGGGGNNCGGANNTTGATTAATTCCATTAATANTAGGAGCCCCAGATATAGCTTTCCCAC GAATAAATAATATAAGATTCTGACTTCTACCACCATCTCTTATATTATTATTAATAAGAAANNNNNATGANNNNNGAGNA GGAACAGGATGAACTTNNNNNCCNCCCCTATCATCAACTATAGGACACAATTCNTCATCNGTAGATTTTGCTATTTTTTC ACTCCNCATTGCAGGAATTTCATCAATCNTNNGAGCAATTAATTTTATTGNAACCATCTTAAATATACATATTAAAACCC ATTCATTANATTTTTTACCTATNTTTACATGATCAGTTTTAATCACAGCAATCTTATTACTATTATCTTTACCAGNTCTA GCAGGAGCTATNACTATACTATTAACTGACCGAAACTTNNNTANNTCATTTNTNTGACNCTNNNGGNAGGAGGANACCCA NTTTTATTTCAACATTTATTNTGANTTTTTGGTCNCCC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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