Sample information |
|
| Picture |
|
|---|---|
| Location | Approximate location: 32.04, -81.17 |
| Collection date | 08/29/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Caught outside of Refresh Nutrition on a semi cloudy and rainy day Caught with my bare hands Many Love bugs were flying around my ignited automobile |
| Putative identification | Arthropoda Insecta Diptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | I was confident that my insect would not be infected with Wolbachia however my results contradicted my prediction. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be postive for Wolbachia. |
GGE-MAN Diptera
KHH-BRI Blattodea
JML-BRI Diptera
CTC-BRI Diptera
VIJ-BRI Hymenoptera