CON-MAR Hemiptera

November 4, 2026

Sample information
Picture
Location
Approximate location: 31.97, -81.16
Collection date 08/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collection sight 3

  • Date: Wednesday 8/26/2026
  • Time: 6:50
  • Location: Outside the Student Union
  • Environment: Beautiful vegetation, many different plants and animal life.
  • Collection Method: sweep net and beat sheet.
  • Results: crickets, ants, rollie pollies(were not kept), lizard (was not kept).

Amazing ground for life, even found an underground bee nest but chose not to catch one. 
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am highly confident that my insect is not infected with Wolbachia after my results from the gel electrophoresis revealed it was absent from my insect.
Wolbachia 16S sequence
Arthropod COI sequence
AATAATTATCCGGGTTGAATTA GCACAGCCAGGATCTCTATTCTTCTCTAATAACAGACAAGTTTACAATGTAATTATTACTTCTCATGCATTCATTATAAT TTTCTTTATAGTTATACCTATTATAATTGGAGGTTTCGGAAACTGACTTCTCCCTCTTATAATTGGAGCACCTGATATAG CATTTCCTCGTCTTAATAATATAAGATTCTGACTTCTACCTCCATCATTATCTCTTTTACTTATAAGATCTATAATTGAA ATAGGAGTGGGAACGGGATGAACTGTTTATCCCCCTTTGTCAAGAAACATTGCCCACGCTGGGCCTAGAGTTGACATATC AATTTTTTCATTACACTTAGCTGGAATTTCATCAATTCTAGGAGCAGTTAATTTTATTACGACTGTAATAAACATACGAC CTACAGGTTTAACACTTCACCAAACACCATTATTTGTTTGATCTGTTTTAATTACAGCAGTTCTTCTATTATTATCCCTT CCGGTACTTGCAGGGGCAATTACTATACTTTTAACAGACCGAAACATCAATACAACATTTTTTGACCCCTCAGGAGGGGG TGACCCTATTCTCTATCAACATTTATTTTGANTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
Report Inappropriate Post