Sample information |
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| Picture |
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|---|---|
| Location | Approximate location: 31.97, -81.16 |
| Collection date | 08/26/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations |
Collection date: August 26th, 2026 Time of collection: 6:06 to 6:30pm Collection methods: Beat net and picked from ground Collection locations: Between the Science Center and College of Science and Mathematics, as well as, near and between the Science Center and Lane Library Habitats of collected insects: Bushes, trees, dirt |
| Putative identification | Arthropoda Insecta Diptera |
Methods |
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| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | A DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | I felt semi-confident with my results of positive Wolbachia because the gel electrophoresis had positive, negative, and NTC groups that turned out correctly and my sample had a clear line where the Wolbachia infected control group also had that line (where Wolbachia was located). |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
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| Summary | The Diptera was found to be postive for Wolbachia. |
CON-JRR Neuroptera
CON-RXB Hymenoptera
CONMED Diptera
CON-MAR Hemiptera
CON-CBS Blattodea