CON-SCA Hemiptera

November 10, 2026

Sample information
Picture
Entry by: Sedonie C-A
Location
Approximate location: 31.97, -81.16
Collection date 08/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Cicada was collected outdoors across from Chick-fil-A at Georgia Southern University Armstrong campus on August 26, 2026
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit other
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I have high confidence in my results because the insect CO1 DNA was successfully amplified, but no Wolbachia DNA was detected in my sample. The positive control showed the expected band, supporting my negative result.
Wolbachia 16S sequence
Arthropod COI sequence
AGGNATAATTGGTACAGCTCTTAGAACCTTAATTCGAATTGAATTAGGTATT NNNNNNTCATTTATTGGTGATGATCAAATTTATAATGTCATTGTTACAGCTCATGCATTTATTATAATTTTTTTTATAGT TATGCCTATTATAATAGGTGGTTTTGGTAATTGGTTAGTACCTTTAATAATTGGAGCTCCTGATATAGCTTTTCCTCGAA TAAATAATATGAGATTTTGACTTCTTCCACCTTCTTTAACTTTATTATTAGTGGGTAGATTAGTTGATAGTGGTGCTGGA ACTGGTTGAACAGTTTATCCACCATTATCAAGATACATGTTTCACTCTGGTTCATGTGTTGATTTAACAATTTTTTCTTT ACATTTGGCAGGTGTATCATCAATTCTAGGAGCTGTAAATTTTATTAGAACAATTTTTAATATACGTTCAACTGGTATAG GTCTTGATAAAACTCCTTTGTTTGTTTGATCAGTTTTGATTACTGCATTTTTATTATTATTATCATTACCAGTTTTAGCT GGTGCAATTACTATATTATTAACTGACCGTAATCTNNNTACATGTTTTTTTGATCCATCTGGAGGGGGTGATCCTATTCT TTATCAACATTTATTTTGANTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
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