K.4.2 fly

February 22, 2022

Sample information
Picture
Photos by: Ethan Z., Legend Y., Gavin K.
Location
Collection date 02/23/2022
Captive / Cultivated? Wild-caught
Group Singapore American School
Observations
  • Wings
  • Black body
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer PBS
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Controls worked, but absence in the sample may not be evident of absence in the whole organism
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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