Sample information
Picture	Photos by: Ethan Z., Legend Y., Gavin K.
Location
Collection date	02/23/2022
Captive / Cultivated?	Wild-caught
Group	Singapore American School
Observations	Hairy and spotted legs Antennae Spots all over body
Putative identification	Arthropoda Hexapoda Insecta Blattodea
Methods
Extraction kit	Instagene Matrix
DNA extraction location	Partial abdomen
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniOne
Buffer	PBS
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Blattodea was found to be negative for Wolbachia.