Sample information
Picture	Photos by: Sawyer J.
Location
Collection date	02/23/2022
Captive / Cultivated?	Wild-caught
Group	River Valley Community College
Observations	Faint color on the outer shell
Putative identification	Arthropoda Hexapoda
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	GelRed
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	All positive and negative result provided the expected result
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Hexapoda was found to be negative for Wolbachia.