Ladybug

April 13, 2022

Sample information
Picture
Photos by: Sawyer J.
Location
Collection date 03/23/2022
Captive / Cultivated? Wild-caught
Group River Valley Community College
Observations
Putative identification Arthropoda Hexapoda Insecta Coleoptera Coccinellidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The gel electrophoresis had no issues with contamination or controls. Each control had the expected outcome.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coccinellidae was found to be negative for Wolbachia.
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