Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/23/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | River Valley Community College |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Coccinellidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The gel electrophoresis had no issues with contamination or controls. Each control had the expected outcome. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coccinellidae was found to be negative for Wolbachia. |
Extraction and Analysis of Wolbachia from Halyomorpha halys
Tick
Roly-Poly
Idaho Beetle
Grammonota