Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/31/2022 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Richmond High School |
| Observations | green, near waters little chilly, around trees and under a wooden table. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | During the DNA extraction step when adding AW 1 buffer to the solution, the solution turned pink |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | The procedure went well but when the solution turned it could have altered the results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman