Woodland ground beetle

April 18, 2022

Sample information
Picture
Photos by: Alex P.
Location
Collection date 03/30/2022
Captive / Cultivated? Wild-caught
Group Richmond High School
Observations

It was very fast and tried to run away. It was under a log in the moist dirt.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

After adding AW2 buffer and centrifuging, my solution turned pink.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am very confident my insect didn’t have Wolbachia because there wasn’t a second line present when I looked at my electrophoresis results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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