|Captive / Cultivated?||Wild-caught|
|Group||Richmond High School|
It was very fast and tried to run away. It was under a log in the moist dirt.
|Putative identification||Hexapoda Insecta Coleoptera|
|Extraction kit||Thermoscientific GeneJet Genomic Purification|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR||Duplex Reaction|
|Gel electrophoresis system||Edvotek Gel Electrophoresis|
|DNA stain||SYBR Safe|
After adding AW2 buffer and centrifuging, my solution turned pink.
|Explanation of confidence level||
I am very confident my insect didn’t have Wolbachia because there wasn’t a second line present when I looked at my electrophoresis results.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Coleoptera was found to be negative for Wolbachia.|