Palmetto Bug Wolbachia

April 27, 2022

Sample information
Picture
Photos by: Rocson L.
Location
Collection date 04/07/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Red-ish-brown, six legs, an inch long, antennae an inch and a half, two sets of wings, external shell, hair plates
Putative identification Arthropoda Hexapoda Insecta Blattodea

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Either there was contamination in the gel, or the DNA was placed incorrectly into the gel.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Both the negative control and the positive control had Wolbachia and the positive did not show arthropod DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Blattodea was found to be negative for Wolbachia.
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