Sample information
Picture	Photos by: Angelina s
Location
Collection date	04/10/2022
Captive / Cultivated?	Wild-caught
Group	Hampton Roads Academy
Observations	big (size of thumb nail) , brown, and has 6 legs, had two sets of wings, has a hard shell
Putative identification	Arthropoda Hexapoda
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR
Gel electrophoresis system	MiniOne
Buffer
DNA stain
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	Low
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Hexapoda was found to be postive for Wolbachia.