April 27, 2022

Sample information
Picture
Photos by: Angelina s
Location
Collection date 04/10/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

big (size of thumb nail) , brown, and has 6 legs, had two sets of wings, has a hard shell
Putative identification Arthropoda Hexapoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be postive for Wolbachia.
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