Tiger Beetle (BM-1)

September 7, 2023

Sample information
Picture
Photos by: Xavier Moore
Location
Collection date 05/02/2022
Captive / Cultivated? Wild-caught
Group Woodford County High School
Observations

Green-blue color, iridescent, six-legged, 2 antennae, stomach a deeper blue than the rest of the body.
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Processed bug DNA was combined with the dye so that Wolbachia could be seen during gel electrophoresis, and the samples were loaded into the gel. The samples were run for ~20 minutes, then photographed and analyzed.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

By analyzing the results, we can see both the insect DNA band and the Wolbachia band as two separate bands.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be postive for Wolbachia.
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