|Captive / Cultivated?||Wild-caught|
|Group||Woodford County High School|
Wings, large eyes, somewhat iridescent, six-legs, long and thin tube-like mouth.
|Putative identification||Hexapoda Insecta Diptera|
|Extraction kit||Instagene Matrix|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
|DNA stain||Fast Blast|
Processed bug DNA was combined with the dye so that Wolbachia could be seen during gel electrophoresis, and the samples were loaded into the gel. The samples were run for ~20 minutes, then photographed and analyzed.
|Explanation of confidence level||
The two different bands are visible, with the bug DNA and Wolbachia bands shown as two separate bands.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Diptera was found to be postive for Wolbachia.|