Bombyliidae sp. AS1

May 19, 2022

Sample information
Picture
Photos by: Annina S.
Location
Collection date 03/29/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

I found this animal near to an old tree trunk, it was slightly above the ground.
Putative identification Arthropoda Hexapoda Insecta Diptera Bombyliidae

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Everything worked well. The extraktion was a bit difficult because I needed to figure out, where the sex organs are located. However the elektrophoresis worked well.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Two bands are quite clearly evident. The upper band proves that it had animal material in the DNA, the lower band proves the Wolbachia in the DNA.
Wolbachia 16S sequence Download FASTA    Download AB1
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCCAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGNNTCNNNCCNAAAGCTA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
NNTNACAGTCATCTNNTCTTGNCGTGAGNTTTNGGGNGTGCCGGGNCNNGNGAGACNGNNNCNTNNNTGCGNTCAANNANGNNNNNNGANNNNGANGCTGCNTNNAAGNNTTAANTTATATNAGNNAGNNAGNTNGGGANNNNTGNTAAGNGNTNTNTTNTNNGGGNGNTNTNGAGNNGANGNNGNAGNNGAGGGNTNNTACNANGCAAGAAAAANNAAAAAANGAANAGAAAGAANGGGGGCGCNANGGAAAGATNAGANANAGAANGAANTNNAAAAGNNGNNGGGAGGGNNNNNNNTNTNCTCTGGNNNNGGACNCNCTGCCCGGCTCGCCATGGGAATTGCTTCNNATTCANNNNANNACNCAGGAANTAACGGTGACNNAGGGNCANNANAAGANNCNNNAACCGTNAAANGACNNGAGGCTGGCGTTTATCCNTAGGCTCNGCCCCCCAGACANNCNTCACGANAANCNAAACTCAACTCANAAGNGNNGAANCCCNNCANNACTATAANNANANAATNCGNTACCNCCTGNNAACTCCCTCNNACGCNCTCCGGNNCAAACCCTGCCNCNTACCCGTATACCTGTCCNCTTACTCCCNTCATGANANTGGCNATTCCCCTNCCTCCGCAGCAGATTGCCACTNNCGNATANGNNTTCCNCNCNANNANNGCTGNGNNANCNNNNNCCCCCNCNCANNCNNNNNNCNNNGCCNNATNNNGCNNCNNNCCNTCCCNNNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Bombyliidae was found to be postive for Wolbachia.
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