Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 05/02/2022 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Woodford County High School |
| Observations | Collected from the area near a dry creek bed with some scattered light and heavy vegetation. Some matured trees surrounded the area. Collected using a malaise trap. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Following the test, we were able to see the separate bands of DNA depicting the insect’s DNA, as well as any further bands of DNA. As you can see in the image, there are two distinct bands, the first of which is the insect’s DNA, allowing us to conclude that the second band present to represents the Wolbachia DNA present in the insect.
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| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be postive for Wolbachia. |
Water Boatman