House Fly (BH-1)

May 24, 2022

Sample information
Picture
Photos by: Xavier M.
Location
Collection date 05/02/2022
Captive / Cultivated? Captive / Cultivated
Group Woodford County High School
Observations

Collected from the area near a dry creek bed with some scattered light and heavy vegetation. Some matured trees surrounded the area. Collected using a malaise trap.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Following the test, we were able to see the separate bands of DNA depicting the insect’s DNA, as well as any further bands of DNA. As you can see in the image, there are two distinct bands, the first of which is the insect’s DNA, allowing us to conclude that the second band present to represents the Wolbachia DNA present in the insect.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be postive for Wolbachia.
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