Sample information |
|
Picture |
|
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Location | |
Collection date | 04/30/2021 |
Captive / Cultivated? | Wild-caught |
Group | KantiWil |
Observations | |
Putative identification | Arthropoda Hexapoda Insecta Hemiptera Pyrrhocoridae Pyrrhocoris Pyrrhocoris apterus |
Methods |
|
Extraction kit | DNeasy (Qiagen) |
DNA extraction location | Abdomen |
Single or Duplex PCR | Duplex Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | SYBR Safe |
Gel images |
|
Protocol notes | |
Results |
|
Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | Co1-band is visble, W-band is not visilbe |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Pyrrhocoris apterus was found to be negative for Wolbachia. |