Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/30/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Formica Formica rufa |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Line 28: Co1-Band is clearly visible, no W-Band |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
TAATTGGTTCATCTATAAGAATAATTATTCGACTAGAATTAGGTTCTTCTAATTCATTAATTAATAATGATCAAATTTATAATTCTATAGTTACAAGACATGCATTTGTTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTTGGAAATTTTCTAGTACCTTTAATATTAGGATCACCTGATATAGCATACCCCCGTATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATTTCTCTCCTTCTTTTAAGAAATTTTATTAATGACGGAGTAGGAACAGGATGAACTGTTTATCCTCCATTAGCTTCTAATATTTTCCATAATGGCCCCTCAGTTGATTTAACTATTTTTTCTCTTCATATTGCTGGTATATCCTCTATTCTAGGAGCTATCAATTTTATTTCAACTATTATAAATATACATCATAAAAATTTTTCTATCGATAAAATTCCCTTACTTGTATGATCAATTTTAATCACTGCAATTTTATTACTTTTATCACTTCCAGTTCTTGCAGGAGCTATCACTATACTTCTAACTGACCGTAACCTTAATACTTCATTTTTTGACCCATCTGGTGGTGGTGATCCTATTTTATATCAACATCTCTTCTG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Formica rufa was found to be negative for Wolbachia. |
Joro Spider
Imported Red Fire Ant
Black carpenter ant
Flocculent tailed millipede
Tawny crazy ant