Sample information
Picture	Photos by: Benoit K.
Location
Collection date	04/30/2021
Captive / Cultivated?	Wild-caught
Group	KantiWil
Observations
Putative identification	Arthropoda Hexapoda Insecta Coleoptera Carabidae Calosoma Calosoma inquisitor
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level	Line 27: Co1-Band is faintly visible, no W-Band
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Calosoma inquisitor was found to be negative for Wolbachia.