Aedes sticticus HA2

Sample information

Picture
Photos by: Anna H
Location
Collection date 09/13/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

forest air humid

Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Aedes Aedes sticticus

Methods

Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

controls worked

Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
CATTAAGAATTTTAATTCGTGCTGAATTAAGTCAACCAGGTATATTTATTGGAAATGACCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTCTTTATAGTAATACCTATTATAATTGGAGGGTTTGGAAATTGATTAGTTCCTTTAATACTAGGAGCCCCTGATATAGCCTTCCCACGAATAAATAATATAAGTTTCTGAATATTACCCCCCTCATTGACACTACTACTCTCTAGTAGTATAGTAGAAAATGGGTCTGGGACAGGATGAACAGTTTATCCGCCTCTTTCATCAGGAACTGCTCATGCCGGAGCTTCTGTTGATTTAACAATTTTTTCTCTTCATTTAGCTGGTGTTTCATCAATTTTAGGAGCAGTAAATTTTATTACAACAGTAATTAATATACGATCAGCAGGTATTACTTTAGATCGATTACCTTTATTTGTTTGATCTGTAGTAATTACAGCTATTTTATTACTTTTATCTTTACCTGTATTAGCTGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACTTCCTTCTTCGACCCTATCGGAGGGGGAGACCCAATTTTATACCAACATTTATTTTGATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Aedes sticticus was found to be negative for Wolbachia.
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