Araneus Diadematus AH2

October 26, 2022

Sample information
Picture
Photos by: Amélie H.
Location
Collection date 09/13/2022
Captive / Cultivated? Captive / Cultivated
Group KantiWil
Observations

By the stone legs of a bench on the side.
Putative identification

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

There was no anthropod DNA detected, although spiders are anthropods. Therefore the confidence level of the spider being Wolbachia negative is low.
Wolbachia 16S sequence Download FASTA    Download AB1
TGCTATAAGAGTATTGATTCGTATTGAATTGGGGCAATCTGGAAGATTGCTGGGGGATGATCAATTGTATAATGTTATTG TTACTGCGCATGCTTTTGTAATAATCTTTTTTATAGTTAT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary
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