Moth (Polites peckius)- Sofia Park Jadotte

November 7, 2022

Sample information
Picture
Photos by: Sofia Park Jadotte
Location
Collection date 09/14/2022
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

active, collected in dirt in the afternoon
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls worked as expected and there were no issues with protocol. Gels and controls were visible as bands.
Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence
NNNNNNANGANCTTCCTNNNNNNNNCTGANTCNANCNNAATTANGNANTCCNGNNNCNNNNNTTGGANATGATNNNNNNNNTNNTACTATTGNNACNGCTCATGCTTTTATTATAATTTTTTTTATANNTATACNNNNTNTNATTGGAGGATTTGGAAATTGATTANNACCTTTAATACTANGANCTCCTGATNTANCTTTCCCCCNAATAAATAATATNNNNNNNTGAATATTACCCCCTTCATTAATATTATTAATTTCAAGAANAATTGTANAAAATGGTGCAGGAACAGGTTGAACAGTTTACCCCCCTTTATCTTCTAATATTGCTCATCNNGGATCTTCTGTTGATTTANCAATTTTTTCTCTTCATTTANCTGGAATCTCTTCAATTTTAGGAGCTATTAATTTTATTACAACAATCATTAATATACGAATTAAAAATTTATCATTTGACCAAATACCTTTATTTGTTTGATCTGNANGAATTACAGCTTTATTGTTACTCTTATCTTTACCTGTTTTAGCANGANCTANTACTATACTACTTACNGANCGAANNTTAAATACTTNTTTTTTTNANCNNGCTGNANNANGGGNNCNNNNTNNATACCAACATTTATTTTGATTTTTTGGTCACCNNNNNNNTTNNAN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lepidoptera was found to be negative for Wolbachia.
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