Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/13/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | Living, rolled up in a ball, very undersized(twig) |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Armadillidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All of my controls worked as expected, and I did not have any problems with the protocol. I was not surprised by any bands on the gel, therefore I am confident my arthropod is not infected with Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Armadillidae was found to be negative for Wolbachia. |
Water Boatman