Sample information

I found this tiger crane fly resting on a surface outside. I collected it at night, near midnight, and it was 67°F.

Methods

DNA extraction: I made sure to crush the arthropod really well. I used the whole arthropod.

Gel Electrophoresis Lanes: For CO1 Arthropod PCR

1- 10Kb ladder

2- The second well is my first sample

3- The third well is my second sample

4- The fourth well is my third sample

5- The fifth well is my fourth sample

6- The sixth well is my negative  Arthropod DNA

7- The seventh well is my positive  Arthropod DNA

8- The eighth well is my positive Wolbachia DNA

9- The ninth well is water, and thus negative overall

Gel Electrophoresis Lanes: For Wolbachia Specific Gene  PCR

1- 10Kb ladder

2- The second well is my first sample

3- The third well is my second sample

4- The fourth well is my fourth sample

5- The fifth well is my third sample

6- The sixth well is my positive Arthropod DNA

7- The seventh well is my negative Arthropod DNA

8- The eighth well is my positive Wolbachia DNA

9- The ninth well is water, and thus negative overall

Results

I’m confident in our results because the controls that were supposed to be positive were positive but our samples were not, indicating that our samples were not positive for Wolbachia.