Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/23/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Lepidoptera Crambidae |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | Midori 1:20'000 |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | Download FASTA
Download AB1
|
| Arthropod COI sequence | Download FASTA
|
| Summary | The Crambidae was found to be negative for Wolbachia. |
Melanoplus femurrubrum
Sehirus cinctus
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus