Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/31/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Antioch High School |
| Observations | I collected it while it was hopping from grass to concrete. |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera |
Methods |
|
| Extraction kit | Wizard SV (Promega) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | We had a lot of errors, and the DNA ran off the gel and when we were putting the DNA in the gel it overflowed. RESULTS INCONCLUSIVE. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Spotted cucumber beetle (Diabrotica undecimpunctata) C-5 SSP
DKH10
DKH 9
DKH8
DKH 7