Black Ant

January 19, 2023

Sample information
Picture
Location No map location data available.
Collection date 08/29/2022
Captive / Cultivated? Wild-caught
Group Antioch High School
Observations

It was small, it was black, it didn’t resist.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Formica

Methods
Extraction kit Wizard SV (Promega)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

I am not sure if it has Wolbachia. The results didn’t turn out as expected.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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