Sample information
Picture	Photos by: Niomee T.
Location
Collection date	03/01/2021
Captive / Cultivated?	Captive / Cultivated
Group	Essex North Shore Agricultural & Technical School
Observations
Putative identification	Arthropoda Entognatha
Methods
Extraction kit	Monarch DNA extraction (NEB)
DNA extraction location
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	Ran gel twice and obtained the same results. This leads me to be confident in my findings.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Entognatha was found to be negative for Wolbachia.