Testing Ticks for Wolbachia

Sample information

Photos by: Ruby S.
Collection date 07/22/2022
Captive / Cultivated? Wild-caught
Group Essex North Shore Agricultural & Technical School

Both ticks appeared to be American Dog Ticks. They were small, brown, and white, and had their legs still attached. Tick 22-95 was measured to be 0.0048g and Tick 22-98 was 0.0044g.

Putative identification Arthropoda Chelicerata Arachnida Ixodida Ixodidae Dermacentor Dermacentor variabilis


Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes
  1. A 1% 60mL gel was made with 1X TBE


Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls ran as expected. Arthropod DNA was confirmed in all tick samples, meaning DNA was collected.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Dermacentor variabilis was found to be negative for Wolbachia.
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