Testing Ticks for Wolbachia

December 13, 2022

Sample information
Picture
Photos by: Ruby S.
Location
Collection date 07/22/2022
Captive / Cultivated? Wild-caught
Group Essex North Shore Agricultural & Technical School
Observations

Both ticks appeared to be American Dog Ticks. They were small, brown, and white, and had their legs still attached. Tick 22-95 was measured to be 0.0048g and Tick 22-98 was 0.0044g.
Putative identification Arthropoda Arachnida Ixodida Ixodidae Dermacentor Dermacentor variabilis

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes
  1. A 1% 60mL gel was made with 1X TBE

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls ran as expected. Arthropod DNA was confirmed in all tick samples, meaning DNA was collected.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Dermacentor variabilis was found to be negative for Wolbachia.
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