Reticultermes tibialis- Common Termite

March 11, 2023

Sample information
Picture
Photos by: Chase Hartzell, Heather Ahlstrom
Location
Collection date 09/06/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations

Common Termite. In a dry area underneath rotten log.

Description: Pale yellow-red hue in a cluster

Body length: .50 cm. Found by Charyn Brooks
Putative identification Arthropoda Insecta Blattodea Rhinotermitidae Reticulitermes Reticulitermes tibialis

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Wolbachia Primers from Wolbachia Project Protocol.
Wolbachia 16S sequence
Arthropod COI sequence
>CO1_5_2_CspecF_cleaned GTGCCTGATCAGGAATGGTTGGAACCTCACTCAGAATACTAATTCGAACAGAATTAGGACAACCCGGATCCTTAATTGGGGATGACCAGATCTACAACGTCATCGTTACTGCCCACGCATTCGTTATGATTTTCTTCATGGTTATGCCAATCATAATTGGTGGATTCGGAAACTGACTAGTACCACTTATATTAGGAGCCCCGGATATAGCATTCCCACGAATAAACAACATAAGATTTTGATTACTCCCACCATCACTAACACTACTACTTACTAGTAGAACAGTAGAAAGTGGGGCAGGGACAGGATGGACAGTTTACCCCCCTCTTGCAAGTGGTATTGCCCATGCAGGAGCATCCGTAGACTTAGCCATTTTCTCCCTACACCTAGCAGGTGTTTCATCAATCCTGGGGGCAGTAAACTTTATCTCAACGACAATTAATATGAAACCAAAAAACATAAAACCCGAGCGAATCCCACTATTTGTATGATCTGTCGCCATTACAGCTCTACTTCTACTACTGTCCCTACCAGTATTGGCCGGAGCAATCACTATGCTATTAACAGATCCCAACCTAAATACATCATTCTTTGACCCGGCCGGAGGGGGGGACCCAATTCTATACCAACACTTATTCTGATTTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Reticulitermes tibialis was found to be negative for Wolbachia.
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