MANACGSp23-21

April 5, 2023

Sample information
Picture
Entry by: Alexandra (Alex) D.
Location
Collection date 01/25/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Collection Date: January 25th, 2023
  • Collection Times: Insect 1 @ 1:26 PM

Insect 2 @ 1:29 PM

Insect 3 @ 1:34 PM

Insect 4 @ 1:35 PM

Insect 5 @ 1:40 PM

  • Location of Collection: GSU Armstrong Campus between the walkways of Annex #2 and between the walkways of Annex #2 and Student Recreation Center across from Science Center by trees and a green electricity distribution box
  • Environment (Habitat) of Collection: Grassland – Subtropical
  • Leafy and a bit wet from rain
  • Method of Collection: Hands (picking up leaves and branches)/Insect Forceps and Collection Pan, Collection Jar
Putative identification Arthropoda Insecta Dermaptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Confidence was low at the end of the experiment due to gel being difficult to see and possible user error made with partner’s sample which had better quality DNA than my sample.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Dermaptera was found to be negative for Wolbachia.
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