|Captive / Cultivated?||Wild-caught|
|Group||Woburn Memorial High School|
|Putative identification||Arthropoda Hexapoda Insecta Hymenoptera Formicidae|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Edvotek Gel Electrophoresis|
Gel was moved a bit after having the DNA injected into the wells.
|Explanation of confidence level||
All positive and negative controls worked, and there is only one band which is closest to the Wolbachia – negative control band.
|Wolbachia 16S sequence||
5'CAT ACC TAT TCG AAG GGA TAG 3' Forward, 5' AGC TTC GAG TGA AAC CAA TTC 3' ReverseBLAST at The Wolbachia Project BLAST at NCBI
|Arthropod COI sequence||
5' GGT CAA CAA ATC ATA AAG ATA TTG G 3' Forward, 5' TAA ACT TCA GGG TGA CCA AAA AAT CA 3' ReverseBLAST at The Wolbachia Project BLAST at NCBI
|Summary||The Formicidae was found to be negative for Wolbachia.|