Ant

Sample information
Picture	Photos by: Austin A. , Dawna W.
Location
Collection date	09/14/2022
Captive / Cultivated?	Wild-caught
Group	Woburn Memorial High School
Observations
Putative identification	Arthropoda Hexapoda Insecta Hymenoptera Formicidae
Methods
Extraction kit	Edwards Buffer
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Edvotek Gel Electrophoresis
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes	Gel was moved a bit after having the DNA injected into the wells. Used a 2% agarose gel instead of a 1%. DNA ladder is in well 1, + DNA control is in well 2, water is in well 3, pillbug is in well 4, Wolbachia + is in well 5, Wolbachia – is in well 6, Ant is in well 7
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	All positive and negative controls worked, and there is only one band which is closest to the Wolbachia – negative control band.
Wolbachia 16S sequence	5'CAT ACC TAT TCG AAG GGA TAG 3' Forward, 5' AGC TTC GAG TGA AAC CAA TTC 3' Reverse BLAST at The Wolbachia Project BLAST at NCBI
Arthropod COI sequence	5' GGT CAA CAA ATC ATA AAG ATA TTG G 3' Forward, 5' TAA ACT TCA GGG TGA CCA AAA AAT CA 3' Reverse BLAST at The Wolbachia Project BLAST at NCBI
Summary	The Formicidae was found to be negative for Wolbachia.

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The Wolbachia Project Database