Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 01/25/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Caught in the field by the greenhouse. |
| Putative identification | Arthropoda Insecta Coleoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | A DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I am confident in my results because I correctly followed the protocols and steps as well as my ability to identify whether or not the bands determined if my insect was infected or not, which it was not. I do have some doubts because the ladder did not transfer into the gel, which might have caused a disturbance. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Bombus impatients (Eastern Bumblebee)
Agelenopis
My Data
Common Eastern Bumble Bee (Bombus impatiens)
American Bird