NESACBSP23-09Hymenoptera

April 10, 2023

Sample information
Picture
Photos by: Lizber P.
Location
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Took safety precautions, turning objects over (pine straw, branch), use hands to dig out dirt, put dirt on collection tray, use forceps to grab insects, place inside collection jar.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Results were inconclusive as there were no bands present for 16S rRNA or CO1.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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