NESACKSP23-5-DN Hymenoptera

April 13, 2023

Sample information
Picture
Photos by: Diago N
Location
Collection date 01/26/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Location: East side of science building, in tall grass, near aquaponic center
  • temp: 46 degrees, windy
  • Under log was moist from previous rainfall
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

All experiments were completed to the best of my ability, with an attempt to make minimal to no mistakes
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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