GRE-ACF-SP23-22 DERMAPTERA

April 19, 2023

Sample information
Picture
Photos by: Saniyya D.
Location No map location data available.
Collection date 01/25/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Time ~ The insects were collected within ten minutes between
1:20 PM.

Location ~ Armstrong campus by the parking lot between the
library and Student Union. Jenkins Hall

Natural Environment ~

They were found below a big tree and bushes in the
ground within soil and leaves.

Warm and gloomy day and clear environment

71 degrees

Method of Collection ~ The insects were first collected by the
sweeping method with the aerial net, but then it became more
effective to use my hands to dig in the soil.
Putative identification Arthropoda Insecta Dermaptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I have a high confidence level for my results being correct because I worked through the entire process as accurate and precise as I could.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Dermaptera was found to be negative for Wolbachia.
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