GRE-ACF-S23#11 Neuroptera

April 19, 2023

Sample information
Picture
Location
Collection date 01/25/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Location: Tea Olive Tree outside science center

Time and Date: 1409 1/25/2023

Weather: 71

Method: Beating
Putative identification Arthropoda Hexapoda

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I have a medium confidence level because even though the gel electrophoresis provided the CO1 gene, without having the sequence its hard to be 100% sure.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
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