Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/25/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Location: Tea Olive Tree outside science center Time and Date: 1409 1/25/2023 Weather: 71 Method: Beating |
| Putative identification | Arthropoda Hexapoda |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I have a medium confidence level because even though the gel electrophoresis provided the CO1 gene, without having the sequence its hard to be 100% sure. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hexapoda was found to be negative for Wolbachia. |
Water Boatman